LOCUS       sequence-342633-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE032
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcca acccgccggg tggtgacccg gaccctggct gccagaccga ctgcaactat
       61 cagcgcggtc cggatccgac cgacgcttat ctggaagctg cctccggccc ctacacggtg
      121 tctacaatcc gcgtatcctc tctggttccg ggtttcggcg gcggtactat ccactacccg
      181 acgaacgctg gtggtggcaa gatggctggc atcgttgtga tccctggtta tctctccttc
      241 gaaagctcca tcgaatggtg gggcccgcgc ctggcgtccc acggcttcgt tgtaatgact
      301 atcgacacca acaccatcta cgaccagcca tctcagcgtc gtgaccagat cgaagcagct
      361 ctgcagtacc tggtcaacca gtccaactct agtagcagcc cgatttctgg gatggttgac
      421 tcttcccgcc tcgcggcagt aggttggtct atgggcggtg gtggcaccct gcaactggct
      481 gctgacggtg gtatcaaagc cgcgattgcc ctggctccgt ggaacagttc tatcaatgat
      541 tttaaccgta ttcaggtacc gaccctgatc ttcgcttgtc agctcgatgc tatcgctcca
      601 gtggcgctgc acgcctcgcc gttctacaac cgcatcccta acaccacgcc gaaagcgttt
      661 ttcgaaatga ccggcggtga ccactggtgc gctaacggcg gtaacatcta tagcgccctg
      721 ctgggaaaat atggcgtgtc ttggatgaaa ctgcacctgg accaagatac tcgttatgct
      781 ccgttcctgt gcggcccgaa ccacgccgct cagaccctga ttagcgaata ccgtggcaac
      841 tgtccttacc tcgag
//
