LOCUS       sequence-342635-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE033
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcat ttgcgatcac tccgtctccg accccaaccc cggatccgac cccgaatcca
       61 tccccggatc cgggctcctg ttccggcgcc gagtgctaca tccgcggtcc taaccctact
      121 gtacgtgccc tggaagcaga cgatggtccg tactcggtgc gtaccaccaa cgtatcttcc
      181 ttcgtttctg gcttcggtgg tggcacaatt cactacccgg tgggtaccga aggcaagatg
      241 ggtgccatcg ccgtgattcc gggctacgtt tcctacgaat catccatccg ttggtggggt
      301 agccgcctgg cgtcatgggg ttttgttgtt attaccatcg acactaacac catttatgat
      361 caaccggatt ctcgtgcaaa ccagctgtca gccgctctgg attacgtgat cgctcaaagc
      421 aactctcgta actcgtccat ttccggcatg gtggactcca accgcctggg tgttatcggc
      481 tggtctatgg gtggtggcgg ttctctgaaa ctgtctactc agcgcacgct gaaagccgca
      541 atccctcagg ctccgtggta ctctggtttc aacagcttca accgcattac tactccaacg
      601 ctcattattg cctgcgagct ggacgttgta gctcctgtag gtcagcacgc ttctccgttt
      661 tacaaccgca ttccgagctc cactgcgaaa gcgtttctgg aaatcaatgg tggcgaccat
      721 ttctgcgcca acagcggcta cccgaacgaa gacatccttg gcaaatatgg cgtttcttgg
      781 atgaaacgct ttattgacgg tgatcgtcgc tacgaccagt tcctgtgtgg tccaaatcac
      841 gaatctgatc gctctatcag cgactaccgt gaaacctgta actacctcga g
//
