LOCUS       sequence-342639-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE035
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcgg ataacccgta tcagcgcggt ccggatccga ctgaacgttc tgttacggct
       61 cgtcgtggcc cgtttgcgat cgacgaaatc tcagttaacg gcggtatcgg tgcaggcttt
      121 aaccgtggta ccatcttcta cccgacggac cgctcccaag gtacgttcgg tgcagttgcc
      181 gttatcccgg gctttctgtc tccggaaagc ctggttcgtt ggttcggccc gcgtctggcg
      241 tcccaaggtt tcgtcgttat gaccttaacg acaaacggcc tgacggacac gccagaatct
      301 cgttctgaac aactgctggc ggctctggat tacctgacta cccgtagcca ggttcgtgac
      361 cgaattgatc cgtcccgtct ggccgttatg ggccattcta tgggtggtgg cggttcgctg
      421 gctgcagctg ccaagcgtcc aaccctgcgt gcagccatcc cgctggctcc gtggtccctg
      481 accaagaact ggagcgacct gaccgtaccg accctgatca tcggtgcaga gaacgataac
      541 gtggcgccgg tcgccggtca ctctgaacgc ttttacgact ccatgaccaa tgtaccggaa
      601 aaagcatact tagaaatggc aggtggtaac catgttgacc cgaccgctga atctgatctg
      661 gtggccaagt tcaccatttc ttggctgaaa cgtttcgttg atgatgatac tcgctatgat
      721 cagttcctgt gtccagctcc gcgtcctaac cgccagatct cagaataccg cgacacctgc
      781 cctcatagcc tcgag
//
