LOCUS       sequence-34264-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P
  TITLE     Efficient construction of sequence-specific TAL effectors for 
            modulating mammalian transcription.
  JOURNAL   Nat Biotechnol. 2011 Feb;29(2):149-53. doi: 10.1038/nbt.1775
  PUBMED    21248753
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-02"; volume: "29"; issue:
            "2"; pages: "149-53"
COMMENT     Sequence Label: pLenti-EF1a-TALE1
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(75..224)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(249..269)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gggccaggat tctcctcgac gtcaccgcat gttagcagac ttcctctgcc ctctccactg
       61 cctctagagt taatcagcat gtccaggtcg aaatcatcaa gggcgtcact gccgagcatg
      121 tcgaggtcaa agtcatcaag ggcatccgaa ccaagcatgt caaggtcaaa atcatcgagg
      181 gcgtcacttc ccagcatatc cagatcaaaa tcgtccaatg cgtcagcccg tccggaaccg
      241 ctggcctcca cctttctctt cttcttgggg ctagctgacg cgcgagtttg gtccccttca
      301 tgcatgggcg agggcgcgtc caaatccctc tccagcgagt ctgcgaacgc atgaaggctc
      361 gcctggtcag gtgtctgagt tgacgtaggt gaaggcttcg cgcgtttcat accgctcgct
      421 tggagaatcc tgtcccaccg ctgggaggcg ggaggcagtg tgcccgagcg ggcctcgagt
      481 tctgtgacac cgactctacg aaagagctgc agcagtccgt gtctcgacat accaaattga
      541 gtcatggcgt catcgaacgc ttgtgcgggg tgggagtgac actggaagaa tccgagcacg
      601 cggaccactt gcgcgtgatc tgccactcga tgtgatgtcc tctcgggaat ccttctgttg
      661 gtccgcttaa tcaatgcagg cgcgtgcggg agccccttct tcaccgcatc caaggcgggt
      721 cgtccgccga ggcaggccag cgctacaaga tgatcattag tgagtgcggc cagcgcgggg
      781 tccggcctcg aaagctgggc cacgattgac tccagtgcgg gtctgccccc gatattggat
      841 gcaatagcca ctacctgctc aggcgtgagt ccgtgcgctt ggcacagcac cggcagcaat
      901 cg
//
