LOCUS       sequence-342641-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE036
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcagg cagataccga taccactgca gtggctcccg cggcggctaa tccgtatgaa
       61 cgcggcccgg ctccgactga agcgtctgta actgcagctc gcggtccgtt tgctattgcc
      121 caggtgaacg taccgtctgg cagcggtgct ggcttcaacg atggcaccat ctactatccg
      181 actgatacct ctcagggtac ctttggtgcg gtcgcggtaa tcccgggttt catctcccct
      241 caggctgtga tccagtggtt cggtccgcgc ttggcatctc agggcttcgt agtcttcact
      301 ctggattcta acggtctggc cgatctgccg gatgcgcgcg gtcgtcagct gctggcggct
      361 ctggactacc tgaccaccca gtctactgtg cgtacccgta ttgatccgaa tcgcctggct
      421 gtcatggggc acagcatggg tggcggtggc acgctgctgg cggcggaaaa ccgtccaacc
      481 ctgaaagcgg ccatcccact ggcgccgtgg gaaccggata ctagttggga aggcgtgaaa
      541 gtaccgacta tgatcatcgg cggcgaaagc gatgtcgttg ctccggtttc cagtatggct
      601 attccggact ataactccct gagctctgct ccagaaaagg cttatctgga gttgcgttct
      661 ggtgatcacc tggcaccggc aagcgaatct cctaccgttg cggaatacgc tttaagctgg
      721 ctcaagcgct ttgttgatga tgacactcgt tatgatcagt tcctgtgtcc gggtcctaca
      781 ccggatactg atatcagcca gtacctggat acgtgtccta acggttctct cgag
//
