LOCUS       sequence-342643-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE037
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcgtt ttcgtgttgc agcgtctctg ccggctgagt atctggctgt ggacaacgtt
       61 gtcctggaag gtaccgctca gccaccggca ccgggtgggt cgggttatca gaaaggccca
      121 gagccaaccg cggccctgct ggaagcgggt accggtccat tcgcaaccgc tagcgttacc
      181 ctgtcccgct ccgcggcctc cggcttcggt gggggtacca tccattaccc gcaaggtgta
      241 gccggccctt tcgcggcagt tgcggtcgtt ccgggctacc tggctgcaga gtcgaccatc
      301 gcttggtggg gtccgcgcct ggccagccac ggtttcgttg ttatcactat ggcgacgaac
      361 aacaccttgg acctgccggc cagccgcagc gctcagctga ctgcagcttt aaaccagtta
      421 aaaaccctgt ccgcaactcc gggtcatgca gtttttggcc tggttgaccc gaaccgcctg
      481 ggtgtggtag gttggtctta cggtggcggt ggtactctgc tgaacgctca ggctaacccg
      541 cagctgaaag cagctatggc cctggctcca aaaaccctcc tccagggtga tttcactggt
      601 accaccgtcc cgacccttgt tgttggttgt caggcggata ctaccgctgc tccggcgttt
      661 tgggcaatcc cattctacaa caaggtgtcc gcttctacgg gcaaagcata cctggaagtt
      721 cgtggtgggt cacacttctg cgtaaccagc agcacctctg atgcagacaa aaaggcgctg
      781 ggtaaatacg gtgtagcgtg gctgaaacgc ttcatggacg aagatacccg ctatgctcca
      841 ttcctgtgcg gtgctccacg ccaggctgac gtcgcaggca atgcggcaat tagcgactat
      901 cgtgataact gcccgtatct cgag
//
