LOCUS       sequence-342645-        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE038
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcgg ataacccgta tcagcgtggc ccggatccga ctcgcgattc tgtcgccgca
       61 tctcgtggca ccttcgctac ggcctccacc accgtaggct ctggcaatgg ttttggtgct
      121 ggcttcatct actacccgac tgacacgtcc cagggtacat ttggcgccgt cgcaatcgtg
      181 ccgggttaca ctgcaacctg ggcagcagaa ggcgcttgga tgggtcactg gctcgcgagc
      241 ttcggttttg tcgtcatcgg catcgatacc atcaaccgca acgactggga cactgcgcgt
      301 ggtacccagc tgcttgccgc gcttgactac ttgactcagc gttcaaccgt tcgtgatcgt
      361 gtggatgctt cccgtcttgc ggttatgggc cactccatgg gcggcggtgg tgcaatgtac
      421 gccgcactgc agcgcccgag tctgaaagct gctgtgggtc tggcaccgtt ctccccgtca
      481 cagaacttga acggtatgcg tgtaccgacg atgctgctgg ccggacaaca cgacaccacg
      541 accacgccgg cgtccatcac cagcctgtac aacggcattc cggcggcaac tgaaaaagca
      601 tacctggaac tgagcggtgc gggccacggc ttcccgacca gcaacaattc tgttatgatg
      661 cgtaaagtaa ttccgtggct gaaaatcttt gtagattcag acgttcgtta tacgcagttt
      721 ctgtgtccgc tgatggataa cactggcatc cgtagctacc agtctacctg tcctctgctg
      781 cccggtaccc cgactccgcc gaaccgttac gaagccgaga cttcgccggc cgtttgtact
      841 ggtactattg ctagcaacca cactggttat tccggtactg gtttttgtga cggtaacaac
      901 gctaccaacg cttacgccca gtttaccgtt aacgcgtctg ccgctggttc aatgaccctg
      961 cgtgtgcgtt tcgcgaacgg taccaccacc gctcgccccg cgagcctgat tgtgaacggc
     1021 agcactgtcc agaccccgtc ctttgaaggc actggcgcgt ggaccacctg ggcaaccaaa
     1081 acactgaccg tgaccctgaa cgccggtaac aacactatcc gtttcaaccc gaccaccgcg
     1141 aacggcctgc cgaaccttga ttacatcgaa attgccgctc cgctcgag
//
