LOCUS       sequence-342647-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE039
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgaaac cgatcacctt caccctgctg ttcattttca tctgctccat tttctattct
       61 caatgcgaag aagtaaacct agaaagcatc agcaactccg gtccgtacgc agttggctct
      121 ctgatcgaag gcgtggatcc gattcgcaac ggtccggatt acgatggcgc caccatctat
      181 tatccgatta acggtacccc accgtactct ggtatcgcaa tcatcccggg ttactgcggc
      241 gttgaaagcg atatccaaga ttggggcccg ttctacgctt ctcacggcat cgtagcaatt
      301 accctgggca ccaacgaccc ttgcgccgac tggccgagcg cacgctccac cgctcttctg
      361 gatgcgatcg ttactgttaa agaagaaaac tcccgccaag attctccgct gaaagacaaa
      421 attgacgtga attctttcgc cgtttctggt tggtctatgg gtggtggtgg cagccagctg
      481 gccgcttcca tcgacccgtc cctgaaggcc gtaattggtc tgtgtccgtg gctggatctg
      541 aacggcttcg aaccgtccga cctgatccac gacgtgccgg ttctgatctt taccggtgaa
      601 aatgacgata ttgctaactc cgcagagtac ggctacatgc attatcaagg caccccgtct
      661 acgaccgata aactgtactt cgagattgcg aacggtggcc atggtgcggc caactctccg
      721 gaattggaag gtggtgaagt cggcgtatac gctctgtctt ggttgaagac ctacctggac
      781 aacgacccgt gctactgcga atttctggtg aacaccccgt ccaactcttc ggattacgaa
      841 accaacatcg aatgtttaaa cgcaggtatt gatgaaggtg aaaacctgat ccacttcatc
      901 tacccgaacc cgatccagga ctacatcgaa ttctctaacg acggcatgga gcgtacttac
      961 gaactcaagt ccagcaacgg taaatctatt aaaagcggca tcgtatccca cggttataac
     1021 aagatcctgt tcgagaaaca gaacactgaa atttactttt taattattgc cggtaaatcc
     1081 tacaaactga tttctattaa actcgag
//
