LOCUS       sequence-342649-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE040
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgggtg attgtccagc aactgctatc tgtcgcagcg aaagcccggg cgcgtactcc
       61 ggtaacggcc cctatggttc tcgctcctac accctgagcc gcttccagac gccgggtggt
      121 gctaccgtgt actatccggc gaacgcagaa ccgccgtacg ctggtatggt ctttaccccg
      181 ccgtataccg gcactcaggc gatgttcgct gcttggggcc cattcttcgc gtctcacggc
      241 ttcgttctgg ttaccatgga cacgagcacc acactggact ccgtcgacca gcgtgctgct
      301 cagcagaaag aagtactgaa cgcactgaaa tctgagaaca cccgttccgg ctctccactg
      361 cgcggtaaac tggataccgc acgtctgggc gctgttggct ggtccatggg tggtggcgca
      421 acttggatca atagcgcaga atactccggc ctgaaaaccg ctatgtctct ggctggtcac
      481 aacctgacgg cagttgatat tgatagcaag ggctataata cccgtgtgcc gaccctgctg
      541 ttcaacggtg cacaggatct gacttacctg ggcggtttgg gccagtctga tggcgtatac
      601 aacaacatcc cggcgggaat cccgaaagtt ttttatgaag tcagcagcgc gggccacttt
      661 gattggggtt ccccgactgc ggccaaccgt tctgtggcgt ctctggcgct tgccttccac
      721 aaagcatacc tggatggcga cacccgttgg ctgcagtaca ttactcgtcc gagcagcgat
      781 gttactactt ggcgtaccgc gaacattcgt ctcgag
//
