LOCUS       sequence-342651-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE041
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgtccc aagtcccgcc aacggatcct caggacgcgc cgttgggcga atgccctgct
       61 accgccttgt gtcgttcaga agcgccgggt tcttacagcg gcaacggtcc gtacggttat
      121 cgcagctatt ccctgtctcg tctgcaaacc ccgggcggcg caaccgttta ttatccggca
      181 aacgcggagc caccgtactc gggtctcgtt ttcacgccgc cgtacaccgg cgtgcaattc
      241 atgtacgccg cgtggggtcc gttttttgcg tcccacggca tcgtactggt gactatggat
      301 accactacta ccctggacac tgttgatcaa cgcgcacgtc aacagaaaac tgtactggat
      361 gttctgaaag gcgaaaacaa tcgtgcagca tcgccgctgc gcggtaaact ggatacctca
      421 cgtattggtg ctgttggctg gtccatgggt ggaggcgcga cctggatcaa tgcagctgaa
      481 tatgcaggtc tgaaaaccgc gatgtctttg gctggccata acctgtccgc tatcgatccg
      541 aatgcgcgtg gctacaacac tcgcgtgccg accttactgt tcaacggtgc actggacgcg
      601 acctacctgg gcggtctggg tcagagcgat ggggtgtata atgcaatccc ggcgggcatc
      661 cctaaggtat tctacgaagt tgccagcgcg gggcatttcg attggggttc ccctaccgcc
      721 gctaaccgtg atgtagcggg tattgcactg gcgttccaca aagcattcct ggacggcgac
      781 acccgctggg tcgattacat ccgccgccct tctcgtgacg ttgcaacttg gcgcaccgca
      841 tacctgccag acctcgag
//
