LOCUS       sequence-342653-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE042
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcgg actgccctgc cggtgcgatc tgccgctatg acgaacagcc gggcggctat
       61 actggtgacg gtccataccg cgtgggtgat tactccatca gcactttcca agcagcgggc
      121 ggtgcgaccg tttactatcc gaccaacgca actccgcctt tcgctgctct ggttttctgc
      181 ccgccgtata ccggcgttca gtacatgtac cgcgactggg gtccattctt cgcgtcgcac
      241 ggcatcgtta tggttaccat ggactccgaa accacgctgg acaccgttga tcaacgtgct
      301 gaccagcagc gtgaagtttt ggactttctg aagcgtgaaa atactaactc ccgctcccct
      361 ctgtatggga aattggctac tgatcgtttt ggtgttacgg gttggtctat gggtggcggc
      421 gctacctgga tcaactctgc cgattacagc ggtcttaaaa ctgcgatgtc cctggccggc
      481 cataacctga ctgccctcga tccggacagc cgtggctatt ccactcgcat cccgactctg
      541 atcatgaacg gtgcactgga tactacctac ctgggtggcc tgggtcagag cgacggcgtc
      601 tataacgcga tcccgtatgg tgtcccgaaa gtgttctacg aagtttcctc tgcaggccac
      661 ttcgcatggg ggtctccgac gtctgcgtcg gacgatgtgg caaaagtagc gctggctttt
      721 cagaagactt tcctggaagg tgacactcgc tgggccgaat acatccgtcg tccgttctgg
      781 ggcgcttctg agtgggagac cgcaaatctg ccgctcgag
//
