LOCUS       sequence-342655-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE043
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgtccc aggttccgcc gaccccgccg accgatgatc cgatgggtga ttgcccgtct
       61 acagctatct gccgaggcga ggcgccgggt agctattctg gtaacggccc gtatggttcc
      121 cggagctaca ccctgtctcg tttccagacc ccgggcggcg caaccgtata ctacccgtct
      181 aacgccgaac caccgtacag cggtctggtt ttcactccgc cgtacaccgg tactcaggct
      241 atgtttcgcg catggggccc attttttgca tctcacggta tcgttctggt aaccatggac
      301 acgtccacta cagtggacac cgttgatcag cgtgcgagcc agcagaaacg cgtactggac
      361 gttctgaaac aggaaaacac gcgttcgggc tctccgctcc gtggtaagct ggacacttcc
      421 cgtctgggtg ccgtgggctg gagtatgggt ggcggagcta cctggatcaa ctctgcggag
      481 tacaacggtc tcaaaacggc tatgagcctc gcaggtcaca atatgaccgc tatcgatctg
      541 gacagcaaag gtggtaacac ccgtgttccg accctcctgt tcaacggcgc gctggacctg
      601 accatgctgg gtggcctggg ccagtctatc ggtgtttaca acgctatccc gcgcggtatt
      661 ccgaaagtta tctacgaagt tgccagcgct gggcacttcg actggggttc cccaaccgca
      721 gcgaatcgtt ccgttgcggg tatcgcactg gcgttccaca aaacgtttct ggatggcgac
      781 acccgttggg tttcctacat caaacgtcca tcctccgatg tggctacctg gcgtaccgaa
      841 aacctgccgc agctcgag
//
