LOCUS       sequence-342657-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE044
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgaaca aggaaaagtc ctctttcgat cagactgcta aaatcaccac ccgctctaaa
       61 tccatcttca aaactatctt cacttacttg ctggtactgg cgttcatcac tacgattttc
      121 ccgatgaacg catttgccaa ctctccagct atcatccgta acgaagaagc gccgggcaaa
      181 tatgcgggga acgggccgtt cagctataat tcctaccgtc tgccactcct ctctgtttat
      241 ggtaccggcg gcgcgaccgt ttactaccct actagcggta ctgcgcctta cagcggtctg
      301 gtgtactgtc cgccatatac ggccaaacaa tcggcactgg cagcttgggg cccgttcttc
      361 gcctcccacg gtatcatcct ggtgactttc gacacgctga ccccgcttga cccggtctcc
      421 ctgcgtgcgc tgcagcagcg taccgttctt aacgcactga aaactgaaaa ctcccgtctg
      481 aatagtccgc tgtaccagaa ggtcgcaacc gaccgtatcg gcgctatggg ctggtccatg
      541 ggcggcggcg caacttggat caactctgcg gagtattcgg gcttgaaaac cgcgatgacg
      601 atcgctggtc acaacctgag ctccaccaac ctgaactcta agggctataa caccaagtgc
      661 ccgaccctga ttatgaacgg tgcgatggac acgaccggcc tgggtggcct gggtcagtct
      721 aacggcgtct acaaaaacat tccagcaaat gtgcctaagg tgttatacga ggttgccagc
      781 gcaggtcatc tgaactggac ttccccgatc tcggcctcta atgatgtggc tgccatcgcg
      841 ctggcgttcc aaaagacgta cctggacggc gactcacgtt ggttggcatt cattactcgt
      901 ccgaacagca acgtgtctat ctgggaaacc agcaacctga tgaacccgct cgag
//
