LOCUS       sequence-342659-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE045
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgtcca acccatacca acgtggtccg aacccgaccc gttctgcctt gaccgccgac
       61 ggtcctttct cagttgctac ctatactgtt agccgtttat ccgtatctgg tttcggtggc
      121 ggcgttattt actatccgac tggtacctcc ctgaccttcg gcggcatcgc gatgagcccg
      181 ggttacaccg ccgatgcttc cagcctggcg tggctgggtc gccgtctggc ttcccacggc
      241 tttgtagttc tggtcattaa caccaactca cgtttcgact acccggactc tcgtgcgtct
      301 cagctgtccg ccgctctgaa ctatctgcgt acgtcatctc cttctgcagt tcgcgctcgt
      361 ctggatgcta atcgtctggc tgtagccggc cacagcatgg gtggtggtgg tacgctgcgc
      421 atcgccgaac agaacccgtc tctgaaagct gcggttccgt tgactccgtg gcataccgat
      481 aaaactttta acacttccgt gccggttctc attgtaggtg ccgaagcgga tactgtcgca
      541 ccagtctccc agcacgcgat cccgttctac cagaacctgc catccactac ccctaaagtg
      601 tatgtagaac tggataacgc atctcacttt gcgcctaact ctaacaacgc ggctatcagc
      661 gtgtacacca tctcgtggat gaaactctgg gttgataacg acactcgtta ccgccagttc
      721 ctgtgtaacg ttaacgatcc agccctgtca gattttcgta cgaacaaccg acactgtcaa
      781 ctcgag
//
