LOCUS       sequence-342661-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE046
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcaga ccagcccgcc gactagcgct agccttaacg cgactgcagg cccgctgtct
       61 gtttcgacct ccagcgtatc tagctgggca gcacgcggtt tcggtggtgg caccatctat
      121 tacccgaacg ctaccggccg ctacggtgtt gtggcaatct caccgggcta tactgcgcgc
      181 cagtcctcaa ttgcttggct gggccgtcgt ctggctaccc atggtttcgt ggtgatcact
      241 attgacacca actccacctt ggaccagccg ccgtcccgtg ctacccagct catggcggca
      301 cttaaccacg tagtgaacaa cgcgaacgct acggttcgtt cgcgtgtcga tgcctctaaa
      361 ctcgcggtag ccggccattc catgggtggt ggcggttctc tgatcgctgc tgaaaacaac
      421 ccgagcctga aagcagccta cccgctgact ccgtggagcg tctctaaaaa ctacagtagt
      481 gttcgtgtgc cgaccatgat catcggcgct gatggtgatt ccatcgcaag tgtgtccacc
      541 cactctcgtc tgttctacaa ctctctgtct agcaacgtta gcaaagcgta cggcgaactg
      601 aacaacgcgt ctcatttcac ccctaactac accaacactc cgattggccg ctacgctgtt
      661 acctggatga aacgtttcgt agacaacgat acccgttatt ctccgttcct ttgcggtgca
      721 ccgcacgaca gctacgcgac ccgtaccgtg ttcgaccgct atgaagataa ctgtgcgtat
      781 ctcgag
//
