LOCUS       sequence-342663-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE047
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggaga gtccgtacga acgtggtccg gacccgactt ctgcatccgt tctggataat
       61 ggaacctttt cactgtcctc cacgtccgtg tcttctcttg tgacgggttt cggtggcggc
      121 accatttatt atccgacctc caccactcag ggcacgtttg gcggcgtagt tttagcaccg
      181 ggctacactg cgagcagctc ctcttattct agcgtggccc gccgcgtggc atctcacggc
      241 tttgtggtct tcgcgattga tactaattcg cgctacgatc agccggatag ccgtggtagc
      301 cagattctgg cggctgtatc ctacctgaaa aactctgcgt cgtccaccgt ggcctcccgc
      361 ttggatgaga cccgtatcgc ggttagcggt cattctatgg gcgggggcgg caccctggca
      421 gccgccaacc aagattcttc catcaaagct gcggtcgcac tgcaaccgtg gcacacggat
      481 aagacgtggc cgggcatcca aatcccgact atgattatcg gcgctgaaaa cgactccgtt
      541 gcgccggtcg ccagccactc tattccgttt tatacttcta tgaccggcgc tcgcgaaaag
      601 gcgtatggtg aaatcaacaa cggtgatcac ttcatcgcta acaccgatga cgactggcag
      661 ggccgtttgt tcgttacctg gctgaaacgc tatgtcgatg atgatacgcg ttactcccag
      721 tttctgtgcc cggcgccgtc ctctatctac ttgtctgatt atcgcaacac ctgtccggat
      781 ctcgag
//
