LOCUS       sequence-342665-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE048
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcagg cgcagtacca gaaaggtccg gatccgactg cttctgctct ggagcgcaac
       61 ggtccgttcg ctatccgttc aaccagcgtt agccgtacta gcgtaagcgg ctttggtggt
      121 ggccgtctgt actacccgac ggccagcggc acgtatggtg cgattgccgt tagccctggt
      181 tttaccggca ctagctctac tatgaccttt tggggtgaac gtctggcctc tcacggcttc
      241 gtagtacttg taatcgatac aatcactctg tacgatcagc cggactcccg cgcacgccag
      301 ctgaaagcag cactggacta cctggccacc cagaacggtc gctcctcatc tccgatctat
      361 cgtaaagtcg acacttctcg tcgtgcggtt gccggccaca gcatgggtgg tggcggcagt
      421 ctgctggcag cacgtgacaa tccatcttac aaagccgcga tcccaatggc gccgtggaac
      481 acctcctcta ccgcctttcg taccgtttct gtcccgacca tgatcttcgg ctgtcaggat
      541 gactctatcg ccccagtatt ctctcatgct atcccgttct acaacgcgat cccgaacagc
      601 acgcgcaaaa actacgttga aatccgtaac gacgaccact tctgtgtgat gaacggcggt
      661 ggccacgatg caactctggg taaattgggc atctcttgga tgaaacgctt cgtggacaat
      721 gatacccgtt acagcccgtt cgtgtgtggt gcggagtaca accgtgttgt ttcatcttac
      781 gaagtgtccc gttcttataa caactgtccg tatctcgag
//
