LOCUS       sequence-342669-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE050
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggctg cgaatccgta ccaacgtggc ccggatccaa ccgaatcgct gctgcgcgcc
       61 gctcgcggtc cgttcgccgt ttcagaacaa tctgtttctc gtttatctgt ctccggtttt
      121 ggtggtggtc gtatctacta tccgaccact acgtcccagg gtacgtttgg cgctatcgct
      181 attagcccgg gttttaccgc atcatggagc tcgctcgctt ggctgggccc gcgcctggcg
      241 agtcatggtt tcgtagttat cggtattgaa accaacaccc gcctggacca gccggattcc
      301 cgtggccgtc agctgctggc tgctctggac tacctgaccc agcgttcctc tgtgcgcaac
      361 cgtgttgacg cgtctcgcct ggcggtcgca ggtcactcca tgggtggtgg cggcactctg
      421 gaagcggcaa agagccgtac cagcctgaaa gctgcaatcc cgattgcacc gtggaacctg
      481 gacaaaactt ggccggaagt tcgcaccccg accctgatta ttggcggtga attggacagc
      541 attgctccgg tcgctaccca tagcattccg ttttacaact ctctgaccaa tgcacgtgaa
      601 aaagcttatc tggaactgaa caacgcgtct cacttttttc ctcagttttc caacgatacc
      661 atggctaaat tcatgatctc ttggatgaaa cgcttcatcg atgacgatac gcgttatgac
      721 cagttcctgt gcccgccgcc gcgtgctatc ggtgatattt cggactaccg tgatacttgt
      781 ccgcacaccc tcgag
//
