LOCUS       sequence-342671-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE051
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggctg ctaacccgta tcagcgtggc ccgaacccca ctgaggcgag catcactgcc
       61 gcgcgcggtc cattcaatac tgcggaaatt accgtttctc gcctgtccgt atccggtttc
      121 ggtggtggca aaatctacta tccaacgacc acctcggaag gtaccttcgg tgctatcgca
      181 atttctccgg gtttcaccgc atactggagc tctctcgaat ggctgggcca ccgtctggct
      241 agccagggct ttgttgtaat cggtatcgaa actaacacta ctttagacca gccggaccag
      301 cgtggccagc agctgctcgc tgcgctggac tatctgaccc agcgctcagc agttcgtgat
      361 cgtgttgatg catctcgtct ggcggtagcg ggtcattcga tgggcggtgg tggttctctg
      421 gaagctgcaa aagctcgtac gagtctgaaa gcggcgattc ctctggcacc ctggaacctg
      481 gacaaaactt ggccggaggt gcgcactccg acccttatta ttggtggtga actggacgcc
      541 gtcgcgccgg tggcgaccca ctctatcccg ttctacaaca gcctgagcaa cgctccggag
      601 aaagcctacc tcgaactgga taacgcgtct cacttctttc cgaatattac caacactcag
      661 atggcgaaat acatgatcgc atggatgaaa cgtttcatcg atgacgatac ccgttacacc
      721 cagttcctgt gcccgcctcc gtctaccggc ctgctgagcg acttttcaga tgcacgtttt
      781 acatgcccga tgctcgag
//
