LOCUS       sequence-342675-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE053
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggctt ccccgtacga acgcgggccg gccccaacct ctgcgatttt ggaagcaagc
       61 cgtggccctt tcgcgacttc gtccatcaac gtttcttctc tgtcagttac cggcttcggt
      121 ggtggtgtaa tttattaccc gaccagcacc gctgaaggca ccttcggcgc cgtcgcgatt
      181 agcccgggtt atactgcttc ttggagtagc ctgtcttggc tgggtccacg tatcgcatct
      241 catggcttcg tggttatcgg tatcgagacc aacacgcgct tggatcagcc tgcttcccgc
      301 ggccgtcagc tgctggctgc tctggactac ctgaccgaac gttcctccgt acgtggccgc
      361 atcgattcgt ctcgtctggc tgtagcgggt cactcgatgg gtggtggcgg ttccttggag
      421 gctgcagcag cccgccctag tctgcaagca gccgtaccgc tggcgccgtg gaatcttgat
      481 aaaacctggt ctgacgtccg tgttccgact ctgatcatcg gtggtgaaac cgattccgta
      541 gcaccggttg caacccattc tattcctttt tacaactcga tcccggcatc tagcgaaaaa
      601 gcttaccttg agcttgatgg cgcatcccac tttttcccgc agaccactaa caccccgacc
      661 gcaaagcaga tggtagcgtg gctgaaacgt ttcgtagatg acgacactcg ttatgaacag
      721 ttcctgtgcc cgggtccttc cggctccgcg atccaggagt accgtaacac ctgtccatct
      781 gcactcgag
//
