LOCUS       sequence-342677-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE054
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggccg cggacaatcc gtacgaacgt ggcccagcgc cgaccgaatc ctcgatcgaa
       61 gcactgcgcg gtccttacgc tgtttcccag acctctgtgt ctcggctggc tgcaactggc
      121 ttcggcggcg gcacgattta ctatccgacc agcaccgcgg acggcacgtt tggtgctgtg
      181 gcaatcagcc cgggtttcac tgccctggaa agctctattt cctggttggg cccgcgtctg
      241 gcgtctcaag gcttcgtggt gtttacgatc gacaccctga ccactgtgga ccagccgggt
      301 tcccgtggtg accagctcct ggccgcgctt gattacctca ctcagcgctc ttctgttcgc
      361 ggtcgcatcg attcctcccg tctgggcgtt atgggtcact caatgggtgg cggcggttcc
      421 ttggaagctg ctaaaacccg tccgagcctc aaagctgcta ttcctatgac cccttggaac
      481 ctggataaga catggcctga gctgaggacc cctactctga tttttggcgc ggatgctgac
      541 accatcgcgc cggtggcgac tcacgcgaaa cctttctata atactctgcc ttcttccctt
      601 gaccgtactt acatcgaact gaacaacgct acccactttg ctcctaacac gtctaacacg
      661 accatcgcta aatactccat ctcgtggctg aaacgtttca tcgacaaaga tacccgctat
      721 gaacagttcc tgtgtccgct gcctcagcgt agccttacca ttgacgaagc gcagggcaac
      781 tgtccgcaca cctccctcga g
//
