LOCUS       sequence-342679-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE055
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgtcca acccgtacga acgcggcccg gcaccaaccg aatcttccgt taccgcggtg
       61 cgcggttatt tcgacaccga tactgacacc gtttcgtctc tggtttccgg tttcggcggg
      121 ggtacgattt actatccgac tgacactagt gaaggtactt tcggcggcgt ggtgatcgcg
      181 ccgggctaca ccgcttcaca gtcatctatg gcatggatgg gccaccgtat tgcgtctcag
      241 ggcttcgttg tatttactat cgatacgatt acgcgttatg atcagccgga ttcacgtggt
      301 cgtcagatcg aagcagctct ggactacctg gtggaagatt ctgatgtagc cgaccgtgtt
      361 gacggcaacc gcctggccgt tatgggtcac tctatgggtg gtggtggcac cctggctgca
      421 gccgaaaacc gcccggaact gcgtgcagct atcccgctga ccccgtggca cctgcagaag
      481 aattggtctg atgttgaagt gccgacgatg attatcggcg ctgaaaatga taccgtggcg
      541 agcgtacgta cccattccat cccgttttac gaatctctgg atgaagatct ggaacgcgcg
      601 tacttggaac tggatggtgc ttcccatttc gctccgaaca tttctaacac cgttatcgca
      661 aaatatagca tctcctggct gaaacgtttc gttgatgaag atgaacgtta cgaacaattc
      721 ctgtgtccgc cgccggacac tgggctgttt tcagacttct ccgattaccg cgactcttgc
      781 ccacatacca ccctcgag
//
