LOCUS       sequence-34268-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Hu S, Yang X
  TITLE     Cellular inhibitor of apoptosis 1 and 2 are ubiquitin ligases for 
            the apoptosis inducer Smac/DIABLO.
  JOURNAL   J Biol Chem. 2003 Mar 21;278(12):10055-60. Epub 2003 Jan 13.
  PUBMED    12525502
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-03-21"; volume: "278(12)"; pages: "10055-60. 
            Epub 2003 Jan 13"
COMMENT     Sequence Label: Flag-cIAP1/pRK5
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        225..243
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     225..242
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     256..275
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      322..331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             331..354
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
      121 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa
      181 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      241 agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc
      301 tcggttctat cgattgaatt cgccgccatg gactacaagg acgacgatga caagggatcc
      361 tctagagtcg accacaaaac tgcctcccaa agacttttcc caggtccctc gtatcaaaac
      421 attaagagta taatggaaga tagcacgatc ttgtcagatt ggacaaacag caacaaacaa
      481 aaaatgaagt atgacttttc ctgtgaactc tacagaatgt ctacatattc aactttcccc
      541 gccggggtgc ctgtctcaga aaggagtctt gctcgtgctg gtttttatta tactggtgtg
      601 aatgacaagg tcaaatgctt ctgttgtggc ctgatgctgg ataactggaa actaggagac
      661 agtcctattc aaaagcataa acagctatat cctagctgta gctttattca gaatctggtt
      721 tcagctagtc tgggatccac ctctaagaat ac
//
