LOCUS       sequence-342681-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE056
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggccg acaatccgta cgaacgtggc ccggcgccga ctaccgcaag catcgaagcc
       61 gctcgtggtc cgtacgctgt gtcccagacc accgtttcta gcctggctgt aactggtttt
      121 ggcggtggta cgatttatta cccaacttct accggtgatg gtaccttcgg tgccattgcg
      181 gtgtccccgg gctacaccgc tacgcagtcc tctatcgcat ggctggggcc gcgccttgcg
      241 agccaggggt ttgtagtatt caccattgac accctgacca ccctggacca accggactca
      301 cgtggccgtc agcttctggc ggcactggac catctcaccc aggtttctag cgtgcgcacg
      361 cgcgtcgacg gttctcgtct gggtgttatg ggccattcca tgggcggcgg tggctctctg
      421 gaagcagcaa aggcgcgtcc gtcgctgcag gcagctattc cgctgactcc gtggaacctg
      481 gacaaaagct ggccggaggt tggcaccccg actctgatcg taggcgcaga tggcgatacc
      541 gttgcgccgg tcgcttctca cgcggaacct ttctactctt ccctgccgtc ctctctggac
      601 cgtgcgtacc tggaactcaa caacgcgact cacttcactc cgaactctag caatactacc
      661 attgcgaagt atggtatctc ttggctgaag cgttttgtgg acaacgacac tcgttacgaa
      721 cagtttctgt gcccgctccc gcagccgagc actaccatcg atgagtatcg cggtaactgc
      781 ccgcatacca gcctcgag
//
