LOCUS       sequence-342683-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE057
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcgg ataacccata tgcgcgcggt ccagaaccga ccaccgcttc tgttgaggcg
       61 gctcgtggtc cgtttgctgt tgcgcagacg tccgtttccc gttacgctgt tagtggcttt
      121 ggtggcggta ccgtatacta cccgacgacc accactgcag gtaccttcgg tgcggtagca
      181 gtgagcccgg gttataccgc tcgtcagagc tccattgcgt ggctgggtcc acgtcttgct
      241 tcacagggtt ttgtggtgat tacgatcgac accctgtcga cctacgacca gccggcgtct
      301 cgtggtgatc agctgcgtgc agcgctggca tacctgactc agcgttctag cgttcgcgcc
      361 cgcatcgacc cgacgcgtct agcggtagtt ggccactcca tgggtggtgg tggcgcgctg
      421 gaagcggcca aagacgatcc gtcactgcag gcggcagtgc cgctgaccgg ctggaacctt
      481 gataaaactt ggccggaagt gcgcacaccg acccttgtaa tcggcgccga agatgacggc
      541 gtagcgccgg tacgttccca ctctgaaccg ttttacgcat ctctgccagc cactctcgat
      601 aaggcatacc tggaattacg cggcgctggc cacctggcgc ctaccgtttc caacactacg
      661 atcgccacct ataccctctc ttggctgaaa cgtttcgttg acgacgacct gcgctatgac
      721 cgtttcctgt gtccggctcc ggctacaagc actgcaattg cggaataccg ttctacgtgc
      781 ccgtatctcg ag
//
