LOCUS       sequence-342685-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE058
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcag acaatcctta ccagcgtggt ccggctccga ccaacgcgtc gattgaggca
       61 acacgtggtc cgtacgcggt gagctctacg tccgtgagct cttggctggt ttccggtttc
      121 gacggcggta ctatttatta tcctactacg accgctgatg gcaccttcgg cgcagtggcg
      181 atctccccgg gttacaccgc ttacgaatcc agcattgctt ggttcggcga acgtctggca
      241 tcccaaggct tcgtggtttt caccttcgat actaacacca ctgtagacca gccggcccag
      301 cgtggtgacc agctgctggc ggctctggat tacttaaccc aacgttcttc cgtgcgttcc
      361 cgtgtggacg cgagccgtct gggcgttatg ggtcattcca tgggcggtgg tggttctttg
      421 gaagcatcta aagaccgtcc gtccctgaag gcggcaatcc cgatgacccc gtggaacact
      481 gataaaacct ggagcgagat ccgtactccg accttgattt tcggtgctga aaacgattcc
      541 gtagctccag tcgcatccca ttccgaaccg ttctactcca ccattccgtc caccaccaac
      601 aaaatgtaca tcgaattaaa cggcgcaagc cactttgctc cgaactcctc taacacgacc
      661 atcgccaaat acagcatcag ctggctgaaa cgtttcctgg ataacgacac tcgttatgac
      721 cagttcctgt gccctctgcc tacttctgcg ctgtacattg aagaatctcg cggtacctgc
      781 ccgctgcgtc tcgag
//
