LOCUS       sequence-342687-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE059
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggttg aagccaccga tgttcacggc ccggacccga cggaagaaac gattaccgct
       61 ccgcgtggcc ctttcgacgt tgagcaggaa tcagtttccc gcttcgaagt tgaaggcttc
      121 ggtggcggta ccatctatta cccgaccgac accaccgacg gtctgtttag cgctgtgtcc
      181 atctccccgg gttatactgg cacccaggaa agcatggcct ggtatggccc acgcctcgca
      241 tcacacggtt ttgtggtttt taccattgat acgatcacca ccacggatca gccggattcc
      301 cgtgcccgcc agctgcaggc atcgctggac catctcgtag acgattcctc cgtacgtgac
      361 cgcgttgacc cggcacgtct gggtgttatg ggccactcca tgggcggcgg tggttcactg
      421 aaagcggctc tggataaccc agcgctgcag gcagcgatcc cactgacgcc gtggcatact
      481 actaaggatt tctccggcgt acgcactcca accctgatca tcggtgctca aaacgatacg
      541 gtggcaccgg ttagccaaca tgcagaaccg ttctacgaaa gtttaccgga cgacccgggc
      601 aaagcgtatc tggaactggc gggcgcgggt cacctggcgc cgaacacccc ggacactacc
      661 attgcgaagt attccctggc gtggctgaaa cgcttcctgg acgatgacac tcgttacgac
      721 caatttctgt gcccgccacc gcaggacgac ccggagatcg cagaacatcg ttctacttgc
      781 ccgtacctcg ag
//
