LOCUS       sequence-342689-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE060
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggccg aacccgctga cgtacacggc ccggacccaa ccgaagaatc catcaccgcg
       61 ccgcgcggcc cgttcgaggt cgacgaagaa tccgttagcc gcctgagcgt gtccggtttt
      121 ggtggcggca ctatctacta ccccacggat acgaccgatg gtctgttctc cgcggtgtct
      181 atttctcccg ggttcaccgg cacacaggaa actatggctt ggtacggccc gcgtctggca
      241 tctcagggtt tcgttgtctt caccattgat accattacca ccaccgatca gccagatagc
      301 cgtgcccgtc agctgcaggc aagcctggac tatctggtta acgactcaga cgtgaaagat
      361 atcatcgatc cggcacgtct gggtgtgatg ggtcactcta tgggtggtgg cggctccctg
      421 aaagcagccc tggataaccc ggcgctgaaa gcggcaatcc cactgactcc gtggcacacc
      481 accaaagact tctccggtgt tcagacgccg accctgatca ttggtgcgca gaacgacacc
      541 gttgcacctg taagccagca cgcaaaacca ttttacgaat ctctgccaga tgatccgggt
      601 aaagcttacc tggaactggc aggtgcttcc caccttgctc cgaacaccga caacaccact
      661 atcgcaaaat tctccatcgc atggctgaaa cgtttcctgg acgatgacac tcgttacgat
      721 cagttcctgt gcccgccgcc ggagaacgac gattctattt ccgactacca gtctacctgc
      781 ccgtacctcg ag
//
