LOCUS       sequence-342691-         537 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE061
FEATURES             Location/Qualifiers
     source          1..537
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgccgg gttttctggg ttcctcctcc aactacgcgt ggatgggtcc gcgtctggct
       61 tctcaaggtt tcattgtgtt tctgatcaac accaatacta ggctcgatac cccgccgcag
      121 cgcggtgatc agctgctggc agcgctggat tggctggttg cttcttctcc gtctgcggta
      181 cgtactcgtc tagatgcgcg tcgcttggcg gttgcgggcc attctatggg tggcggcggc
      241 gcgctggaag ctagcctgga tcgcccgtct ctgcaagcaa gcctgccgct gcagccgtgg
      301 cacaccccgg cttctttcag cggcgtacag gttccgacta tgatcattgg tgctgaggcc
      361 gatactaccg cgcccgtagc atcgcatgct gaaccgtttt acgaatctct gacctcggct
      421 tctgaccgtg cctacctgga actcaacggt gcagaccacc gcgtgtcaac cacctctagt
      481 accacccagg cgaaattcat gatcgcctgg ctgaaacgtt tcgtggataa cctcgag
//
