LOCUS       sequence-342693-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE062
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcga acccgtatga acggggtccg aaccctacgg acgctctgct ggaagcacgt
       61 agcggtccgt ttagtgtttc cgaggagaac gtttctcgcc tttctgcttc cggttttggc
      121 ggcggtacca tctactaccc gcgtgaaaac aacacgtatg gtgctgttgc tatcagcccg
      181 ggttatactg gtactgaagc ttccattgct tggctgggta aacgtatcgc tagccacggt
      241 tttgtagtca tcaccatcga taccatcact accctcgatc agccagatag ccgtgcggaa
      301 cagctgaacg cggcactgaa ccacatgatc aaccgtgcgt cgtcgaccgt tcgttctcgt
      361 attgactctt cccgcctggc ggtaatgggc cactctatgg gtggtggtgg ctcgcttcgc
      421 ttagcctctc agcggccgga tctcaaggca gctattccgc tgaccccgtg gcacttaaac
      481 aaaaactggt ctagcgttcg tgtaccgacc ctgatcatcg gcgcggacct ggatactatt
      541 gcgccggttc tgacccacgc gcgcccgttc tacaattcgc tgccgacctc catctctaaa
      601 gcatacttgg aactggacgg tgcgacgcac ttcgcgccga acattccgaa caagattatc
      661 ggcaaatact ccgtggcttg gctgaaacgt ttcgtagaca acgatactcg ttacacacag
      721 ttcctgtgtc cgggtccgcg tgatggtctg tttggtgaag ttgaagaata ccgctccacc
      781 tgcccgtttc tcgag
//
