LOCUS       sequence-342695-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE063
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggctg caaacccgta tgaacgcggt ccgaatccga ccgacgcact gttagaagcg
       61 cgatctggtc cattctccgt atcagaggaa aatgtgtccc gtctgtccgc gtcgggcttc
      121 ggcggtggca ccatttacta cccgcgtgaa agtaacacct atggcgctgt agctatctcc
      181 ccgggctata ctggtaccga agcgtctatt gcatggctgg gtgaacgtat cgcatcccat
      241 ggttttgtag ttattactat tgacaccatt actacgctgg atcaaccaga ctcacgtgct
      301 gagcagctga acgcagcgct caatcacatg attaaccgcg catcgagcac cgtgcgttct
      361 cgcatcgata gctctcgtct ggcggtgatg ggtcactcca tgggtggcgg tggcacgctg
      421 cgtctggcaa gccagcgtcc ggatctcaaa gcagcgattc cgctgactcc atggcatttg
      481 aacaaaaact ggagctctgt gaccgtgccg accctgatca tcggcgccga tctggacacc
      541 atcgcaccgg tggccactca tgccaaacca ttctataact ccctgccgtc atctatctcc
      601 aaggcttacc tggaactgga cggtgcgacc cacttcgctc caaacatccc gaacaagatt
      661 atcggtaaat attcagtagc atggctgaaa tggttcgttg ataacgatac ccgttacact
      721 cagttcctgt gtccgggtcc gcgcgacggt ctgttcggcg aagtggaaga gtaccgttcg
      781 acctgtccgt ttctcgag
//
