LOCUS       sequence-342697-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE064
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcca acccgtacga acgcggtcca aacccgaccg acgcgcttct tgaggcccgt
       61 agcggtccat tcagcgtaag cgaagaaaac gtgtcccgcc tgagcgcctc tggttttggt
      121 ggtggcacca tctactatcc gcgcgaaaac aacacatacg gtgcggtcgc tatctcccca
      181 ggttataccg gtaccgaagc atccatcgca tggcttggtg aacgcattgc aagccatggc
      241 tttgtcgtca tcacgattga tacgatcacc actctggacc agccggattc ccgcgcggaa
      301 cagctgaacg cggctctcaa tcacatgatc aaccgtgcgt cctctaccgt acgttcgcgt
      361 atcgacagct cgcgcctggc tgttatgggc catagcatgg gtggcggcgg ttcgcttcgt
      421 ctggcttcgc agcgtccgga cttgaaggcc gcaatcccac tgaccccgtg gcacctgaat
      481 aaaaattgga gctccgttcg tgtgccgacc ctgatcatcg gtgcggatct ggacaccatc
      541 gcgccggttc tgactcacgc gcgcccattc tacaactctc tgccgacctc tatctccaaa
      601 gcataccttg aactggacgg cgcgacccac ttcgctccga acattcctaa caaaatcatc
      661 ggcaagtata gcgtagcctg gctgaaacgc ttcgtggaca acgatacccg ctacacccag
      721 ttcctgtgcc cgggtccgcg cgacggcctg ttcggcgaag tagaagaata tcgctctacc
      781 tgccctttcc tcgag
//
