LOCUS       sequence-342699-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE065
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcga acccttatga acgtggcccg aacccgaccg atgcgctgct ggaagctcga
       61 tctggtccat tctctgtgtc tgaagaaaac gtgtcccgcc tgggcgcgag cggttttggc
      121 ggtgggacta tctattatcc gcgcgaaaac aacacctacg gagctgttgc gatctctccg
      181 ggttatacgg gcacccaggc atccgtggcg tggcttggca aacgtatcgc aagtcacggg
      241 ttcgtagtca tcaccatcga caccatcacc acgctggacc agccggattc gcgtgcgcgt
      301 cagcttaacg cggcactgga ctatatgatc aatgacgcgt cctccgcagt tcgcagccgc
      361 atcgacagct cccgcctggc ggtcatgggc catagcatgg gcggtggtgg ctctctgcgt
      421 ctggcgtctc agcgtccgga cctgaaagcg gccatcccgc tgaccccgtg gcatctcaac
      481 aaaaactggt cttctgtgcg tgtgccgact ctgatcatcg gggctgatct ggataccatc
      541 gcgccagtac tgacccacgc ccgtccattc tacaacagcc tcccgacgag catcagcaag
      601 gcttacctgg aactggacgg tgctacccat ttcgcaccga acatcccgaa caaaattatc
      661 ggtaagtact ctgttgcttg gttgaaacgc tttgttgaca acgatacccg ttacacccag
      721 ttcctgtgcc cgggccctcg tgacggcctc tttggtgaag ttgaagaata tcgttctacc
      781 tgtccattcc tcgag
//
