LOCUS       sequence-34270-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Tang J, Qu LK, Zhang J, Wang W, Michaelson JS, Degenhardt YY, 
            El-Deiry WS, Yang X
  TITLE     Critical role for Daxx in regulating Mdm2.
  JOURNAL   Nat Cell Biol. 2006 Aug . 8(8):855-62.
  PUBMED    16845383
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Aug . 8(8):855-62."
COMMENT     Sequence Label: Flag-Daxx/pRK5
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        167..185
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     167..184
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     198..217
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             273..296
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg attccccgtg
       61 ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg gcttcgttag
      121 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
      181 atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
      241 cctcggttct atcgattgaa ttcgccgcca tggactacaa ggacgacgat gacaagggat
      301 ccgccaccgc taacagcatc atcgtgctgg atgatgatga cgaagatgaa gcagctgctc
      361 agccagggcc ctcccaccca ctccccaatg cggcctcacc tggggcagaa gcccctagct
      421 cctctgagcc tcatggggcc agaggaagca gtagttcggg cggcaagaaa tgctacaagc
      481 tggagaatga gaagctgttc gaagagttcc ttgaactttg taagatgcag acagcagacc
      541 accctgaggt ggtcccattc ctctataacc ggcagcaacg tgcccactct ctgtttttgg
      601 cctcggcgga gttctgcaac atcctctcta gggtcctgtc tcgggcccgg agccggccag
      661 ccaagctcta tgtctacatc aatgagctct gcactgttct caaggcccac tcagcc
//
