LOCUS       sequence-342701-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE066
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcta acccatacga acgcggtccg aatccgacgg acgccctgct ggaggcgcgt
       61 tctggtcctt tcagcgttag cgaagaacgt gcatcccgtt tcggtgctga tggcttcggt
      121 ggtgggacca tctactaccc gcgtgaaaac aacacatacg gcgcggtcgc tatctccccg
      181 ggctatacgg gcacacaagc ttctgtggct tggctgggtg agcgtatcgc gtctcatggc
      241 ttcgttgtca tcacgattga cactaacacc accctggacc agccggattc acgtgcccgt
      301 cagctgaacg cagcgctcga ttacatgatt aacgatgcct cgtccgctgt gcgttcccgt
      361 atcgattctt ctcgtctggc agttatgggt cactctatgg gtggcggcgg tacactgcgc
      421 ctcgccagcc agcgtccgga cctgaaggct gccatcccac tgaccccgtg gcacctgaac
      481 aaaaactggt cttcagtacg cgtgccgact ctgatcatcg gtgctgacct ggacaccatc
      541 gcgccggttc tgactcatgc gcgtccgttc tacaactctc tgccgacctc tatttcgaaa
      601 gcctatttag agctggatgg tgcaacccac tttgcaccga acatccctaa caaaattatt
      661 gggaagtatt ctgttgcatg gctgaaacgc ttcgtggaca acgacacccg ctatactcag
      721 tttctgtgtc cggggccgcg cgacggtctt ttcggtgagg ttgaagaata ccgttcgact
      781 tgcccgttcc tcgag
//
