LOCUS       sequence-342703-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE067
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcta acccgtacga acgtggcccg aacccgaccg atgcactcct ggaagctcgc
       61 agcggtccgt tctcggtttc ggaggaacgt gcgagccgct tcggtgcaga tggtttcggc
      121 ggtggcacca tctactaccc gcgcgaaaat aacacttatg gcgcagtggc gatttcgccg
      181 ggttacaccg gtacccaggc atccgtggca tggctgggta agagaattgc aagccacggt
      241 ttcgtagtta ttactatcga taccaacacc actctcgatc agccagattc tcgcgcgcgc
      301 cagctgaacg cagccctcga ctacatgatc aacgatgcgt cttctgcggt gcgtagccgc
      361 attgacagct ctcgtttggc agtaatgggc cactctatgg gcggcggtgg gtctctgcgt
      421 ctggcttctc agcgtccgga cctgaaagct gcaatcccac tgacgccgtg gcacctgaac
      481 aaaaattggt ctagcgtccg tgtgccgacc ctgatcatcg gtgcggatct ggatactatt
      541 gcaccggtgc tgacccacgc ccgcccgttc tataacagcc tgccgacctc catttcaaaa
      601 gcttacctgg agctggatgg tgccacccac ttcgctccaa acatcccgaa caaaattatc
      661 ggtaaatatt ctgtcgcgtg gctgaaacgt ttcgttgaca acgatacccg ctatactcag
      721 ttcctgtgcc cgggtccgcg tgatggcctg tttggtgagg ttgaagaata tcgctctact
      781 tgtccttttc tcgag
//
