LOCUS       sequence-342705-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE068
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcta acccatacga acgtggtccg aacccaactg acgcactgct ggaagctagc
       61 tccggcccgt tttccgtgtc cgaagaaaac gtttctcgtc tgagcgcttc cggcttcggc
      121 ggtggcacca tctactatcc gcgtgagaac aacacttatg gcgccgtagc catctccccg
      181 ggctacaccg gtactgaagc ttcaatcgca tggctgggtg gtcgtatcgc cagccacggt
      241 ttcgttgtta tcaccatcga tactatcacc acactggatc agccggattc tcgcgctgaa
      301 cagctgaacg cggccctgaa ccacatgatt aatcgcgcct cctccactgt tcgttcccgc
      361 attgattcct cccgcctggc ggtgatgggc catagtatgg gtggtggcgg aaccccgcgt
      421 ctggcctctc agcgcccgga tctgaaagcg gcgattcctc tgaccccgtg gcacctgaac
      481 aaaaaccgtt cctctgtgac tgttccgacc ctgatcatcg gcgcggacct ggataccatc
      541 gctccggtag ccactcacgc taaaccgttc tacaatagcc tgccgtctag catctccaaa
      601 gcttacctag agctggatgg tgcgacccac tttgcgccga acattccgaa taaaatcatc
      661 ggtaaatata gcgttgcatg gctgaaacgt ttcgttgata acgatacccg ctacacccag
      721 tttctgtgtc cgggcccacg tgacggtctg ttcggcgaag tcgaagaata ttgctctacc
      781 tgcccgttcc tcgag
//
