LOCUS       sequence-342707-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE069
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcta acccgtatga gcgtggtccg aacccgacgg aaagcatgct cgaggctcgt
       61 agcggcccgt tttctgtaag cgaagaacgt gcatctcgtc tgggtgcgga tggcttcggc
      121 ggcggtacca tctattatcc gcgtgaaaac aacacgtatg gtgctattgc aatttcccct
      181 ggttataccg gtactcagtc ttccattgcg tggctgggcg aacgtattgc aagccacggc
      241 tttgtggtaa tcgcgatcga caccaacacc acccttgacc agccggactc tcgtgctcgt
      301 cagctgaacg ctgctttgga ttacatgctg accgatgcat cttcctccgt tcgtaaccgt
      361 atcgacgctt ctcgtctggc ggtaatgggc cattccatgg gcggcggtgg cacgctgcgt
      421 ctggcaagtc agcgcccaga cctgaaagca gcgattccac tcactccgtg gcacctgaac
      481 aagtcctggc gtgatatcac cgttccgacc ctgatcatcg gtgcggacct ggacaccatt
      541 gctccggttt ccagccatag cgaaccattt tataactcca tcccgagctc cactgacaaa
      601 gcgtaccttg aactgaataa cgccacccat ttcgcgccga acattaccaa caaaacgatc
      661 ggtatgtaca gtgtggcctg gctgaaacgt ttcgttgacg aggatacccg ctacactcag
      721 ttcctgtgcc cgggtccgcg caccggcctg ctgagcgatg ttgacgagta ccgttctact
      781 tgcccgttcc tcgag
//
