LOCUS       sequence-342709-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE070
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcca acccatatga acgtggtcca aaccctacgc aggcgttact ggaggcacgt
       61 agtggtccat tcagcgtttc cagcgaacgt gcttggcgcc tgggcagcga cggtttcggc
      121 ggtggcacga tttactaccc gcgcgaaaac aacacctacg gtgcggtggc catcagcccg
      181 ggctataccg gtacccaggc ttctgtagcg tggctgggtg aacgtattgc gtcccacggc
      241 ttcgtggtga tcacgatcga taccaatact accctggatc agccggactc tcgtgctcgc
      301 cagctggacg ctgcattaga tcacatgctg aacgacgcta gttccgcggt ccgctctcgt
      361 atcgaccgta accgtttggc ggtaatgggt cactctatgg gtggtggcgg tacccttcgc
      421 ctggcgagcc agcgcccaga cctcaaggct gcaatccctc tgacgccgtg gcacctgaat
      481 aagagctggt ctaatgtcca ggttccaact ctcattattg gggcggacct cgacacgatc
      541 gcgccggtac tgacccacgc agaaccgttc tataactcaa tcccgaccag cacccgtaaa
      601 gcatatcttg aactcgatgg tgccacccac tttgcaccga acatcaccaa ctctaccatc
      661 ggcatgtatt ccgttgcgtg gcttaaacgt tttgtggatg aagacacccg ttacacccaa
      721 ttcctgtgcc cgggcccacg caccggtctc ttttctgacg tagaagaata ccgttctacc
      781 tgcccgttcc tcgag
//
