LOCUS       sequence-342711-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE071
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcta acccgtacga acgtggcccg aacccaacca acagcagcat cgaagcactg
       61 cgaggtccgt tccgcgtcga cgaagaacgc gtctctcgtc tgcaagctcg tggtttcggc
      121 ggcggcacta tctactaccc gactgataac aacaccttcg gcgctgttgc tatctcgccg
      181 ggttataccg gcacccagtc gtctattagc tggctgggcg agcgtctggc gtcccacggt
      241 ttcgtcgtca tgactatcga tactaacacc accttggacc agccggactc tcgtgcatct
      301 cagctggatg cggcactgga ttatatggtg gaagattcta gctattctgt acgtaaccgt
      361 attgattctt ctcgtctggc ggcgatgggt cattccatgg gtggtggtgg taccctgcgt
      421 ctggctgaac gtcgcccgga cctgcaagct gcaatcccgc tgaccccttg gcatacagac
      481 aaaacttggg gctctgtacg tgtaccgacg ctgatcattg gtgccgaaaa tgataccatt
      541 gcatctgtac gcagccatag cgaaccgttc tataatagcc tgccgggatc tctggacaaa
      601 gcgtatctcg aactggacgg cgcaagccac ttcgctccga acctttccaa cactacgatc
      661 gcgaaatact ccatctcctg gctgaaacgt ttcgtggatg atgacactcg ctacacccag
      721 ttcctgtgcc caggcccgtc aactggctgg ggctcagacg tagaagaata tcgcagcact
      781 tgcccgtttc tcgag
//
