LOCUS       sequence-342713-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE072
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcga acccgtacga gcgtggtccg gacccgactc aggcgtccct ggaagcctct
       61 cgtggcccgt tcccggtttc tgaagagcgt gtttcttctc cagtaagcgg cttcgggggc
      121 ggcacaattt attacccgca ggaaaacaac acctacggcg cggtggcaat ctctccgggc
      181 tatactgcta cccagtcctc tgtggcttgg ctgggagaac gcattgcatc acacggcttt
      241 gttgttatca cgatcgacac caacaccact ctggaccagc cggattcgcg tgcagaccaa
      301 ctggaagctg cgctggatca catggtagat ggcgcgtcct ctaccgttcg ctctcgcatc
      361 gaccgtaacc gcctggcagt aatgggtcat agcatgggtg gcggcggtac tctgcgcctg
      421 gcatctcgtc gcccggatct gaaagcggcg atcccgctga ccccatggca cctgaacaaa
      481 agctggtcca acgttcaggt ccctaccctg atcattggcg ccgagaatga cacggttgcc
      541 ccggtagcac tgcacgcgga accgtcctac acctccatcc caacctccac ccgtaaagct
      601 tatctggaac tgaacggtgc gtctcacttt gcgccgagtg tcgctaacgc tactattggc
      661 atgtacggtg ttgcgtggct gaaacgcttt gtcgatgaag acacacgtta cacccgtttc
      721 ctgtgtcctg gtccgcgtac cggcctgttc tccgatgtgg aagaataccg tagcacttgc
      781 ccattcctcg ag
//
