LOCUS       sequence-342717-         372 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE074
FEATURES             Location/Qualifiers
     source          1..372
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcta acccgtacga acgtggtccg aacccgacca actcttcgat cgaagcgctg
       61 cgtggtccgt tccgtgtcga tgaagaacgt gtttcccgtc tgcaggcgcg tggttttggc
      121 ggtggcacta tctactatcc gactgataac aacaccttcg gcgctgtggc tattagcccg
      181 ggttatactg gcacccagtc cagcatctcc tggctgggtg aacgtctggc gtcacacggt
      241 tttgtggtga tgaccatcga taccaacact actctggatc agccggactc tcgcgcgtct
      301 cagctggacg cagcgctgga ttacatggtt gaggattctt cgtactccgt acgtaaccgc
      361 atcgatctcg ag
//
