LOCUS       sequence-342719-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE075
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcga acccttacga acgcggtccg aacccgaccg atgccctgct cgaagctcgc
       61 tcgggcccgt tctctgtctc cgaagaaaac gtgagccgtc tgtcggcttc cggctttggc
      121 ggtggcacaa tttactatcc tcgcgagaac aacacctacg gtgctgttgc gatctctccg
      181 ggctatactg gtacagaggc ttccatcgcc tggctgggcg agcgcatcgc ttctcacggt
      241 ttcgttgtca ttaccatcga tactattacc accctggacc agccggactc gcgtgctgaa
      301 cagcttaatg cagcgcttaa ccatatgatc aatcgtgctt cgtcaaccgt tcgcagccgt
      361 atcgattctt ctcgtctggc ggtgatgggt cattctatgg gtggcggtgg ttcgctccgt
      421 ctggccagcc agcgcccgga tctgaaagcg gcaatcccgc tgactccgtg gcatctgaac
      481 aaaaactggt cttcggttac cgtgccgacc ctgattatcg gtgcagacct ggacacgatt
      541 gcaccggttg cgactcacgc aaaaccgttc tacaactccc tgccgtcttc tatttctaag
      601 gcataccttg aactggacgg tgcaacccat ttcgctccga acattccgaa caaaatcatc
      661 ggtaaataca gcgtggcctg gctgaaacgt tttgttgaca acgacacccg ttacacacag
      721 ttcctgtgcc cgggtccgcg tgacggtctt ttcggcgagg tggaagaata tcgtagcacc
      781 tgtccattct acctcgag
//
