LOCUS       sequence-342721-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE076
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcca acccgtatga acgtgggccg aatccgaccg atgctctgct ggaagcaagc
       61 agcggcccgt tcagcgtttc ggaagaaaac gtttctcgtc tgtctgcgag tggcttcggc
      121 ggcggtacta tctactatcc acgtgaaaac aatacctacg gcgcagtcgc gatttctccg
      181 ggttatactg gcacggaagc atcaattgcg tggctgggtg aacgtattgc gagtcatggt
      241 ttcgttgtta ttactatcga cactatcacc accctggacc agccggacag ccgcgcagag
      301 cagctgaacg ctgctcttaa ccacatgatc aaccgtgcgt cctccaccgt tcgcagccgc
      361 atcgactcat cccgtttggc tgttatgggc catagcatgg gcggtggcgg caccctgcgt
      421 ctggcgagcc agcgtccgga tctgaaagct gcaatcccgt tgactccatg gcatctgaac
      481 aagaactggt cctccgttac ggttccgacc ctgattatcg gcgcggatct cgataccatc
      541 gcaccagttg ccactcatgc aaaaccgttc tacaactcac tgccgagctc gatctctaaa
      601 gcttacctgg aactggatgg cgcgacccat tttgcgccga acattccgaa caagatcatc
      661 ggcaaatact ccgttgcctg gctgaagcgt ttcgttgata atgacactcg ttatacgcag
      721 ttcttgtgcc cgggtcctcg tgacggcctg tttggcgagg tggaagagta tcgttcaacc
      781 tgtccgttcc tcgag
//
