LOCUS       sequence-342723-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE077
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcga acccatacga acgtggtccg aacccgaccg atgcgctgtt ggaagctcgt
       61 agcggtccgt tcagcgtttc tgaggaaaac gtgagccgtt tcggcgcaga cggtttcggc
      121 ggcggcacta tctactaccc tcgcgaaaat aacacatatg gcgcggttgc tatttctccg
      181 ggctacaccg gtacccaagc gagcgttgcg tggctgggcg agcgtatcgc gtcacacggc
      241 ttcgtggtaa tcacaatcga cacaaacact actctggacc agccggattc acgtgcccgc
      301 cagcttaacg cggcgctgga ttacatgatc aacgacgcgt cctcagcggt gcgttcacgt
      361 atcgacagct cccgtctggc ggtgatgggt cactccatgg gcggtggtgg caccctccgc
      421 ctggcgtccc agcgtccaga tcttaaagcg gcaatcccgc tgacaccttg gcacctgaac
      481 aaaaactggt cctctgttcg cgttccaact ttgatcatcg gcgccgatct ggacactatc
      541 gcaccggtgc tcacccacgc tcgcccgttt tataacagcc tgccaacttc gatttccaaa
      601 gcctacctgg aattagacgg tgcgacccac tttgcgccga acattccaaa caaaatcatc
      661 ggtaaatatt ctgtcgcctg gctcaaacgc ttcgtggata acgatactcg ttacacgcaa
      721 ttcctgtgtc caggtccacg tgatggtctt ttcggcgaag ttgaagagta ccgtagcact
      781 tgcccgttcg cactggaact cgag
//
