LOCUS       sequence-342725-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE078
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatggcga acccgtacga acgcggcccg aacccgaccg aatctatgct ggaagctcgt
       61 agcggtccat tctccgttag cgaggaacgt gcttctcgct ttggcgctga cggtttcggt
      121 ggtggcacta tctattatcc gcgtgaaaac aacacctacg gcgcgattgc catctctccg
      181 ggttataccg gcactcagag ctccatcgcg tggctgggcg aacgtatcgc ctcccacggc
      241 ttcgttgtta tcgctattga caccaatacc accctggacc aacctgactc tcgtgctcgt
      301 cagctgaacg cggcgctgga ctatatgctt actgacgcct cctctgcagt acgtaaccgt
      361 attgacgcgt cacgcctggc tgtgatgggt cacagcatgg gtggcggtgg gactctgcgc
      421 ctggcatcgc agcgcccgga tctgaaagcg gctatcccgt taactccgtg gcacctgaac
      481 aaatcttggc gcgacatcac ggtaccgacc ctgatcatcg gtgcagagta tgacaccatc
      541 gcctccgtga cgctgcattc taaaccgttc tacaactcga ttccgtctcc gaccgacaaa
      601 gcttatctcg aactggacgg ggcatctcac tttgcaccga atatcaccaa taagactatc
      661 ggtatgtact ctgtggcctg gctgaaacgt ttcgttgatg aggatacgcg ttacacccag
      721 ttcctgtgtc cgggcccgcg taccggcctg ctgtctgatg tagaagagta ccgttccact
      781 tgcccattcc tcgag
//
