LOCUS       sequence-342729-        2161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of histone fused GCaMP8m under the synapsin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2161)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-Syn-H2B-jGCaMP8m-WPRE
FEATURES             Location/Qualifiers
     source          1..2161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..469
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             503..880
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             896..913
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1195..1216
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1322..1343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1583..1602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg
       61 gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca
      121 aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc
      181 actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg
      241 ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc
      301 cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg
      361 cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc
      421 tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgaattcaag
      481 ctgctagcaa ggatccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
      541 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
      601 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
      661 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
      721 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
      781 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
      841 cgagggtact aaggccatca ccaagtacac cagcgctaag gacccaccgg tcatgcatca
      901 tcaccatcat cacacgcgtc gcaagaagac cttcaaggag gtggccaccg ccgtgaagat
      961 catcggccgc ctgatgggcc tgaagatcaa cgtctatatc aaggccgaca agcagaagaa
     1021 cggcatcaag gcgaacttcc acatccgcca caacatcgag gacggcggcg tgcagctcgc
     1081 ctaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1141 ctacctgagc gtggagtcca aactttcgaa agaccccaac gagaagcgcg atcacatggt
     1201 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     1261 cggtaccgga gggagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1321 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggtga
     1381 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1441 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1501 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacatcca
     1561 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1621 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1681 caacatcctg gggcacaagc tggagtacaa cctgccggac caactgactg aagagcagat
     1741 cgcagaatac aaagaggctt tctccctatt tgacaaggac ggggatggga caataacaac
     1801 caaggagctg gggacggtga tgcggtctct cggacagaac cccacagaag cagagctgca
     1861 ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct
     1921 gacaatgatg gcaagaaaaa tgaaatacag ggacacggaa gaagaaatta gagaagcgtt
     1981 cggtgtgttt gataaggatg gcaatggcta catcagtgca gcagagcttc gccacgtgat
     2041 gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca gggaagcaga
     2101 catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagta
     2161 a
//
