LOCUS       sequence-342732-        2142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  jGCaMP8f expression in GABA-ergic interneurons under the control of 
            mDlx enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2142)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-mDlx-jGCaMP8f-WPRE
FEATURES             Location/Qualifiers
     source          1..2142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          691..823
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     806..825
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             877..894
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1176..1197
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1303..1324)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1564..1583)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatacactca cagtggtttg gcatatattt ggtgaaattt tttaaggaaa aattagtgtt
       61 ggtttcgata tatggtagct ttttctctaa cataatttga ataattcagc aaagccctac
      121 taccagctgt acttctgcag cctcttccat tctttccagc attataattt tggttaattt
      181 tcaattttag gtcctacgtc tctgcaattt gtgtatgaat aacagaataa tttccctctt
      241 ttgtttcgcc tttcctgttc ctgaatctaa ataaagatgg ctttttagta ttaaaagtgg
      301 aagaaaatta caggtaatta tctttgacgg taaaaacgct gtaatcagcg ggctacatga
      361 aaaattactc taattatggc tgcatttaag agaatggaaa aaaaccttct tgtggataaa
      421 aaccttaaat tgtccccaat gtctgcttca aattggatgg cactgcagct ggaggctttg
      481 ttcagaattg atcctgggga gctacgaacc caaagtttca cagtaggaag cttgatatcg
      541 aattcctgca gcccgggctg ggcataaaag tcagggcaga gccatctatt gcttacattt
      601 gcttctagcc tgcaggtcga ggagcgcagc cttccagaag cagagcgcgg cgccttaagc
      661 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      721 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      781 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      841 aattacagcg ctagcaagga tccacccgcc accatgcatc atcaccatca tcacacgcgt
      901 cgcaagaaga ccttcaagga ggtggccacc gccgtgaaga tcatcgcccg cctgatgggc
      961 ctgaagatca acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc
     1021 cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
     1081 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtggagtcc
     1141 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1201 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     1261 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1321 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
     1381 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1441 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1501 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
     1561 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1621 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1681 ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaatt taaagaggct
     1741 ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct ggggacggtg
     1801 atgcggtctc tcggactgaa ccccacagaa gcagagctgc aggacatgat caatgaagta
     1861 gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat ggcaagaaaa
     1921 atgaaataca gggacacgga agaagaaatt agagaagcgt tcggtgtgtt tgataaggat
     1981 ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct tggagagaag
     2041 ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg ggatggtcag
     2101 gtaaactacg aagagtttgt acaaatgatg acagcgaagt aa
//
