LOCUS       sequence-342764-       11205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hFXR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran
            DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler 
            U, Tuschl T
  TITLE     FMRP targets distinct mRNA sequence elements to regulate protein 
            expression.
  JOURNAL   Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 
            2012 Dec 12.
  PUBMED    23235829
REFERENCE   2  (bases 1 to 11205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11737"; journalName: "Nature"; date: "2012-12-20- 
            20"; volume: "492"; issue: "7429"; pages: "382-6"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhFXR2
FEATURES             Location/Qualifiers
     source          1..11205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             759..782
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             783..809
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    825..849
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2921..3020)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3132..3151)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3143..3952
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3210..3229)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4042..4630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      4795..4881
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4973..5000
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     5008..5031
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    5050..5149
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6725..6749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6802..6843
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6923..6940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6929..7153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7286..7305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7437..7484
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7492..8514
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7492..8514
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8644..8765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8681..8700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8735..8754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8814..8830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8814..8830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8827..8849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8838..8854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8862..8868,8869..8886,8887..8892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8907..8928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9045..9062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9216..9804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9975..10766,10767..10835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10598..10617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10836..10940)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11015..11034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccatgga ctacaaggac gacgatgaca
      781 agtaccctta tgacgtgccc gattacgctt tgaaccagat atcaacaagt ttgtacaaaa
      841 aagcaggctc caccatggga accaattcag tcgacatggg cggcctggcc tctggggggg
      901 atgtggagcc gggactgccc gtcgaggtgc gcggctccaa cggggccttc tacaagggct
      961 ttgtgaagga tgtccatgaa gactctgtca ccatcttctt tgaaaacaac tggcagagtg
     1021 agagacaaat tccttttggg gatgtccggc taccacctcc agctgactat aataaggaga
     1081 tcacagaagg ggatgaagtg gaggtttatt ctcgagccaa tgaacaggaa ccttgtggct
     1141 ggtggctggc ccgggtgcgg atgatgaagg gagatttcta tgtcattgaa tatgctgcct
     1201 gtgatgccac ctacaatgaa attgttaccc tggagcgact tcggccagtt aatcccaatc
     1261 cccttgcaac caaaggcagc ttcttcaagg ttaccatggc tgtgcccgag gatctgagag
     1321 aagcctgctc caatgaaaac gtccataaag agttcaagaa agccctggga gccaactgca
     1381 tctttctcaa catcacaaac agtgagctct tcattctgtc aaccacagaa gcccctgtga
     1441 agcgagcatc tctgctgggt gatatgcatt tccgaagcct gcgcaccaaa ctgctactta
     1501 tgtcccgcaa tgaagaagct accaagcacc tagagacaag caagcagttg gcagcagcct
     1561 tccaagagga gttcacagtg cgagaggacc tgatgggact ggcaattggg actcacggtg
     1621 ccaacatcca gcaggcccga aaagtacctg gggtgaccgc cattgagttg ggtgaagaga
     1681 cctgcacttt ccgcatctat ggggagactc ccgaggcttg ccgacaggcc cgaagctacc
     1741 ttgagttttc tgaggactca gtgcaagtgc ccaggaacct ggttggcaaa gtgattggaa
     1801 agaacgggaa agtgatccag gagattgtgg ataaatctgg tgtggtgagg gttcgagtgg
     1861 aaggtgataa tgacaagaag aaccccaggg aggagggaat ggttcccttc atttttgttg
     1921 gcacccgaga gaacatcagc aatgcccagg ctttgctgga gtatcacctc tcctacctgc
     1981 aggaggtaga gcagcttcgc ttggagaggc tacaaattga tgagcagctt cggcagattg
     2041 ggctgggctt tcgccctcct gggagtgggc ggggcagcgg tggcagcgac aaggctggat
     2101 atagcactga tgagagctcc tcctcctccc tccatgcgac tcgaacctat gggggcagct
     2161 atgggggccg tggccgtggc cggaggacag gcggtcctgc ctatggcccc agctcagatg
     2221 tgtctacagc ttcagagact gagtcagaga agagagagga gcccaaccga gctgggcctg
     2281 gcgacaggga tcccccaacc cgaggggaag aaagccggag gcggccgact gggggccggg
     2341 gtaggggacc cccacctgcc ccccggccca cttcgagata caattcttca tctattagct
     2401 cagtgctgaa ggatccagac agtaatccct acagcctatt ggacacgtct gaaccagagc
     2461 ccccggttga ttcagaacct ggggaacccc ccccagcaag tgccaggcgc cgccgctccc
     2521 gccgccgccg cactgatgaa gacaggaccg tcatggatgg aggcctggaa tcagatgggc
     2581 ccaacatgac agagaatggc ctggaagatg aatcaagacc tcaacgtcgt aatcgcagcc
     2641 gccgccgccg taaccgtggt aatcggactg atggctctat cagtggagac cgccagccag
     2701 tgactgtggc tgactatatc tcacgagcag agtctcagag ccgccagagg ccacccctgg
     2761 aacgcactaa accctcagaa gactctcttt caggacagaa gggtgactct gtcagcaagc
     2821 ttcctaaggg cccctcggag aatggggagc tctccgcccc cttggagttg ggtagtatgg
     2881 tgaatggggt ttcataagcg gccgcactcg agatatctag acccagcttt cttgtacaaa
     2941 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3001 caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc aaaatctctg
     3061 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     3121 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     3181 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     3241 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     3301 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     3361 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg gtgatgcatg
     3421 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     3481 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     3541 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     3601 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     3661 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     3721 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     3781 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     3841 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3901 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     3961 ggttaattgg ttgtaacatt attcagattg ggccccgttc cactgagcgt cagaccccgt
     4021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4381 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4441 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4501 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4561 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4621 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4681 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgctag
     4741 catggatctc ggggacgtct aactactaag cgagagtagg gaactgccag gcatcaaata
     4801 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     4861 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
     4921 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
     4981 atcctgacgg atggcctttt tgcgtttcta caaactcttc ctgttagtta gttacttaag
     5041 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
     5101 gataagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc caccatggga
     5161 accaattcag tcgacatggg cggcctggcc tctggggggg atgtggagcc gggactgccc
     5221 gtcgaggtgc gcggctccaa cggggccttc tacaagggct ttgtgaagga tgtccatgaa
     5281 gactctgtca ccatcttctt tgaaaacaac tggcagagtg agagacaaat tccttttggg
     5341 gatgtccggc taccacctcc agctgactat aataaggaga tcacagaagg ggatgaagtg
     5401 gaggtttatt ctcgagccaa tgaacaggaa ccttgtggct ggtggctggc ccgggtgcgg
     5461 atgatgaagg gagatttcta tgtcattgaa tatgctgcct gtgatgccac ctacaatgaa
     5521 attgttaccc tggagcgact tcggccagtt aatcccaatc cccttgcaac caaaggcagc
     5581 ttcttcaagg ttaccatggc tgtgcccgag gatctgagag aagcctgctc caatgaaaac
     5641 gtccataaag agttcaagaa agccctggga gccaactgca tctttctcaa catcacaaac
     5701 agtgagctct tcattctgtc aaccacagaa gcccctgtga agcgagcatc tctgctgggt
     5761 gatatgcatt tccgaagcct gcgcaccaaa ctgctactta tgtcccgcaa tgaagaagct
     5821 accaagcacc tagagacctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     5881 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     5941 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     6001 tcttgccatc ctatggmwcw gmykygkyka swgcttcaga gactgagtca gagaagagag
     6061 aggagcccaa ccgagctggg cctggcgaca gggatccccc aacccgaggg gaagaaagcc
     6121 ggaggcggcc gactgggggc cggggtaggg gacccccacc tgccccccgg cccacttcga
     6181 gatacaattc ttcatctatt agctcagtgc tgaaggatcc agacagtaat ccctacagcc
     6241 tattggacac gtctgaacca gagcccccgg ttgattcaga acctggggaa ccccccccag
     6301 caagtgccag gcgccgccgc tcccgccgcc gccgcactga tgaagacagg accgtcatgg
     6361 atggaggcct ggaatcagat gggcccaaca tgacagagaa tggcctggaa gatgaatcaa
     6421 gacctcaacg tcgtaatcgc agccgccgcc gccgtaaccg tggtaatcgg actgatggct
     6481 ctatcagtgg agaccgccag ccagtgactg tggctgacta tatctcacga gcagagtctc
     6541 agagccgcca gaggccaccc ctggaacgca ctaaaccctc agaagactct ctttcaggac
     6601 agaagggtga ctctgtcagc aagcttccta agggcccctc ggagaatggg gagctctccg
     6661 cccccttgga gttgggtagt atggtgaatg gggtttcata agcggccgca ctcgagatat
     6721 ctagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcaagt
     6781 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     6841 acgcgtacct gatccgcggc cgcatagata actgatccag tgtgcctcga gtctagaggg
     6901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     7201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     7441 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     7501 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     7561 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     7621 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     7681 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     7741 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     7801 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     7861 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     7921 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     7981 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     8041 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     8101 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     8161 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     8221 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     8281 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     8341 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     8401 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     8461 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     8521 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8581 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8641 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8761 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8821 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8881 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8941 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9001 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9061 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9121 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9181 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    10981 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    11041 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
    11101 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
    11161 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
