LOCUS       sequence-342797-       11497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of myosin light chain (Myl9) tagged with 
            mScarlet-I and cytoplasmic iRFP as a reference marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11497)
  AUTHORS   Hadjitheodorou A, Bell GRR, Ellett F, Shastry S, Irimia D, Collins 
            SR, Theriot JA
  TITLE     Directional reorientation of migrating neutrophils is limited by 
            suppression of receptor input signaling at the cell rear through 
            myosin II activity.
  JOURNAL   Nat Commun. 2021 Nov 16;12(1):6619. doi: 10.1038/s41467-021-26622-z.
  PUBMED    34785640
REFERENCE   2  (bases 1 to 11497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-16"; volume: "12(1):6619. doi"; pages: " 
            10.1038/s41467-021-26622-z"
COMMENT     Sequence Label: pLV_iRFP_P2AT2A_mScarlet-I_Myl9
FEATURES             Location/Qualifiers
     source          1..11497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2899..3110
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3111..4049
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4006..4026
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4067..4999
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             5018..5074
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5084..5137
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5147..5839
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(5277..5295)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5545..5564
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6462..7011
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6606..6623)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6833..6852
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    7466..8054
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7519..7539)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7937..7948)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8261..8894
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9022..9038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9022..9038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9035..9057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9046..9062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9070..9076,9077..9094,9095..9100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9115..9136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9253..9270)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9424..10012)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9504..9523)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10183..10974,10975..11043))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10806..10825
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11044..11148)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11196..11330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11233..11252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11287..11306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cacatatgcg ggaggtggtc
     2221 cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt
     2281 tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt
     2341 ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga
     2401 cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa
     2461 gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg
     2521 aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca
     2581 ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga
     2641 ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc
     2701 ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg ggcgcgcgcc
     2761 cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc cccggcggcc
     2821 ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta ggcgcggaga
     2881 ctcgaggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2941 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     3001 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     3061 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     3121 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     3181 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     3241 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaagcctggc
     3301 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     3361 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     3421 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     3481 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3541 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3601 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3661 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3721 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3781 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3841 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3901 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3961 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     4021 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg agcgctatgg cgcgtaaggt
     4081 cgatctcacc tcctgcgatc gcgagccgat ccacatcccc ggcagcattc agccgtgcgg
     4141 ctgcctgcta gcctgcgacg cgcaggcggt gcggatcacg cgcattacgg aaaatgccgg
     4201 cgcgttcttt ggacgcgaaa ctccgcgggt cggtgagcta ctcgccgatt acttcggcga
     4261 gaccgaagcc catgcgctgc gcaacgcact ggcgcagtcc tccgatccaa agcgaccggc
     4321 gctgatcttc ggttggcgcg acggcctgac cggccgcacc ttcgacatct cactgcatcg
     4381 ccatgacggt acatcgatca tcgagttcga gcctgcggcg gccgaacagg ccgacaatcc
     4441 gctgcggctg acgcggcaga tcatcgcgcg caccaaagaa ctgaagtcgc tcgaagagat
     4501 ggccgcacgg gtgccgcgct atctgcaggc gatgctcggc tatcaccgcg tgatgttgta
     4561 ccgcttcgcg gacgacggct ccgggatggt gatcggcgag gcgaagcgca gcgacctcga
     4621 gagctttctc ggtcagcact ttccggcgtc gctggtcccg cagcaggcgc ggctactgta
     4681 cttgaagaac gcgatccgcg tggtctcgga ttcgcgcggc atcagcagcc ggatcgtgcc
     4741 cgagcacgac gcctccggcg ccgcgctcga tctgtcgttc gcgcacctgc gcagcatctc
     4801 gccctgccat ctcgaatttc tgcggaacat gggcgtcagc gcctcgatgt cgctgtcgat
     4861 catcattgac ggcacgctat ggggattgat catctgtcat cattacgagc cgcgtgccgt
     4921 gccgatggcg cagcgcgtcg cggccgaaat gttcgccgac ttcttatcgc tgcacttcac
     4981 cgccgcccac caccaacgcg gaagtgcagg cagcggcgcc acgaacttct ctctgttaaa
     5041 gcaagcagga gacgtggaag aaaatcccgg tcctggctcc ggcgagggca gaggaagtct
     5101 gctaacatgc ggtgacgtcg aggagaatcc tggcccaacg acgcgtgtga gcaagggcga
     5161 ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg gagggctcca tgaacggcca
     5221 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     5281 gctgaaggtg accaagggtg gccccctgcc cttctcctgg gacatcctgt cccctcagtt
     5341 catgtacggc tccagggcct tcatcaagca ccccgccgac atccccgact actataagca
     5401 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgccgt
     5461 gaccgtgacc caggacacct ccctggagga cggcaccctg atctacaagg tgaagctccg
     5521 cggcaccaac ttccctcctg acggccccgt aatgcagaag aagacaatgg gctgggaagc
     5581 gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag ggcgacatta agatggccct
     5641 gcgcctgaag gacggcggcc gctacctggc ggacttcaag accacctaca aggccaagaa
     5701 gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag ttggacatca cctcccacaa
     5761 cgaggactac accgtggtgg aacagtacga acgctccgag ggccgccact ccaccggcgg
     5821 catggacgag ctgtacaagg gagccggtat gtccagcaag cgggccaaag ccaagaccac
     5881 caagaagcgg ccacagcggg ccacatccaa tgtcttcgca atgtttgacc agtcccagat
     5941 ccaggagttt aaggaggctt tcaacatgat tgaccagaac cgtgatggct tcattgacaa
     6001 ggaggacctg cacgacatgc tggcctcgct ggggaagaac cccacagacg aatacctgga
     6061 gggcatgatg agcgaggccc cggggcccat caacttcacc atgttcctca ccatgtttgg
     6121 ggagaagctg aacggcacgg accccgagga tgtgattcgc aacgcctttg cctgcttcga
     6181 cgaggaagcc tcaggtttca tccatgagga ccacctccgg gagctgctca ccaccatggg
     6241 tgaccgcttc acagatgagg aagtggacga gatgtaccgg gaggcaccca ttgataagaa
     6301 aggcaacttc aactacgtgg agttcacccg catcctcaaa catggcgcca aggataaaga
     6361 cgactaagcg atgcatgtcg ggccgcggta acaattgtta actaacttaa gctagcaacg
     6421 gtttccctct agcgggatca attccgcccc ccccccccct aacgttactg gccgaagccg
     6481 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     6541 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     6601 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     6661 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     6721 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     6781 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     6841 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     6901 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     6961 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggtca
     7021 tgaaaacatt taacatttct caacaagatc tagaattagt agaagtagcg acagagaaga
     7081 ttacaatgct ttatgaggat aataaacatc atgtgggagc ggcaattcgt acgaaaacag
     7141 gagaaatcat ttcggcagta catattgaag cgtatatagg acgagtaact gtttgtgcag
     7201 aagccattgc gattggtagt gcagtttcga atggacaaaa ggattttgac acgattgtag
     7261 ctgttagaca cccttattct gacgaagtag atagaagtat tcgagtggta agtccttgtg
     7321 gtatgtgtag ggagttgatt tcagactatg caccagattg ttttgtgtta atagaaatga
     7381 atggcaagtt agtcaaaact acgattgaag aactcattcc actcaaatat acccgaaatt
     7441 aacccgggcg gggcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     7501 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7561 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7621 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7681 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7741 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7801 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7861 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7921 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7981 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     8041 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     8101 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     8161 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     8221 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcgctcc
     8281 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     8341 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     8401 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     8461 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     8521 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     8581 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     8641 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     8701 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8881 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8941 gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag
     9001 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgt gtattgggcg
     9241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11161 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
    11221 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    11281 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    11341 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11401 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11461 attgtatttg ttaaatatgt actacaaact tagtagt
//
